Anticancer Activity of Phanera semibifida Extract Evaluated by Brine Shrimp Lethality Test (BSLT) and Molecular Docking Studies

Meyla Suhendra; Berry  Juliandi; Huda Shalahudin  Darusman; Siti  Sadiah; Fitmawati Fitmawati; Puji  Rianti

doi:10.56303/jhnresearch.v5i1.850

Authors

Meyla Suhendra Program of Animal Bioscience, Department of Biology, IPB University, Indonesia
Berry Juliandi Program of Animal Bioscience, Department of Biology, IPB University, Indonesia
Huda Shalahudin Darusman School of Veterinary Medicine and Biomedical Science, IPB University, Indonesia
Siti Sadiah School of Veterinary Medicine and Biomedical Science, IPB University, Indonesia
Fitmawati Fitmawati Department of Biology, Universitas Riau, Indonesia
Puji Rianti Program of Animal Bioscience, Department of Biology, IPB University, Indonesia

Keywords:

Cytotoxicity, Cyanidanol, Ellagic Acid, Lingga Island, Medicinal Plants

Abstract

Indonesian people use plants as medicine because they are considered to have fewer side effects. The Lingga island community used Phanera semibifida (Roxb.) traditionally to make therapeutic drugs. P. semibifida has the potential to be a medicinal plant. We extracted stems and leaves with three different solvents and tested their cytotoxic activity. The ethanolic stem extract showed the highest value of LC₅₀ in the moderate toxicity category. The ethanolic steam extract continued to be subjected to in silico analysis, using molecular docking. We selected four compounds for molecular docking studies against five cancer-related proteins. The proteins are Estrogen receptor α, CDK2, Human Cytochrome P450 CYP17A1, Cyclin A, and BCL-2. The result showed that Ellagic acid bound strongly to Estrogen receptor α, CDK2, CYP17A1, and Cyclin A protein. Cyanidanol showed the highest affinity to BCL-2. These results suggest that P. semibifida contains compounds that can target multiple cancer-related proteins. Our findings highlight its potential as a source of anticancer agents. We recommend isolating these compounds and testing their efficacy through in vitro and in vivo studies to confirm their therapeutic potential in future studies.

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