Development of Omega-3 and Antioxidant-Rich Bread Spread from Smoked Mackerel and Bambara Groundnut for Cardiovascular Health
Keywords:
antioxidant, bambara groundnut, bread spread, mackerel, omega-3Abstract
Bread spreads can be defined as products that can be spread on bread or as a filling, usually used to add flavor or texture to bread. The taste of bread spread is not only sweet but can be spicy and savory. The purpose of this research was to develop a bread spread made from smoked mackerel with the addition of bambara groundnut and analyze the effect of different formulas on nutrient content, antioxidant activity, and omega-3. This study used a completely randomized design. The analysis test used was One-Way ANOVA with Duncan multiple range test further test with SPSS version 26 and Microsoft Excel 2016 software. The results of this study showed that the highest content of moisture, protein, fat in F1, ash content in F2, while carbohydrates, energy, IC50, and omega-3 in F3. The results of the analysis on bread-spread products contained moisture 31.57-41.06%, ash 4.56-5.00%, protein 15.84-26.82%, fat 28.30-36.24%, carbohydrate 32.14-51.31%, IC50 is 2.66-4.32-ppm, and omega-3 of 1.68-2.6 g/100g. The conclusion of the research showed that the treatment of the ratio of mackerel and bambara groundnut affected on the moisture, protein, and carbohydrate (p<0.05), but did not affect on the ash, fat, omega-3, and antioxidant activity (p>0.05).
Downloads
References
Olvera Lopez E, Ballard BD, Jan A. Cardiovascular Disease. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 [cited 2025 Nov 18]. Available from: http://www.ncbi.nlm.nih.gov/books/NBK535419/
WHO. Cardiovascular diseases (CVDs) [Internet]. 2023 [cited 2025 Nov 18]. Available from: https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds)
Wong ND. Epidemiological studies of CHD and the evolution of preventive cardiology. Nat Rev Cardiol. 2014 May;11(5):276–89.
Kemenkes RI. Riset Kesehatan Dasar (Riskesdas). Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan RI; 2018.
Casas R, Castro-Barquero S, Estruch R, Sacanella E. Nutrition and Cardiovascular Health. Int J Mol Sci. 2018 Dec 11;19(12):3988.
Okafor JNC, Jideani VA, Meyer M, Le Roes-Hill M. Bioactive components in Bambara groundnut (Vigna subterraenea (L.) Verdc) as a potential source of nutraceutical ingredients. Heliyon. 2022 Mar 1;8(3):e09024.
Khan SU, Lone AN, Khan MS, Virani SS, Blumenthal RS, Nasir K, et al. Effect of omega-3 fatty acids on cardiovascular outcomes: A systematic review and meta-analysis. EClinicalMedicine. 2021 Aug;38:100997.
Jiang H, Wang L, Wang D, Yan N, Li C, Wu M, et al. Omega-3 polyunsaturated fatty acid biomarkers and risk of type 2 diabetes, cardiovascular disease, cancer, and mortality. Clinical Nutrition. 2022 Aug 1;41(8):1798–807.
Weinberg RL, Brook RD, Rubenfire M, Eagle KA. Cardiovascular Impact of Nutritional Supplementation With Omega-3 Fatty Acids: JACC Focus Seminar. J Am Coll Cardiol. 2021 Feb 9;77(5):593–608.
Siswanti S, Agnesia PY, A RBK. Pemanfaatan Daging dan Tulang Ikan Kembung (Rastrelliger kanagurta) dalam Pembuatan Camilan Stik. Jurnal Teknologi Hasil Pertanian. 2017 Feb 25;10(1):41–9.
Damayati DS, Jastam MS, Faried NA. Analisis Kandungan Otak-Otak Ikan Kembung (Rastrelliger Brachyoma) Subtitusi Buah Lamun (Enhalus Acoroides) Sebagai Alternatif Perbaikan Gizi di Masyarakat. Al-Sihah: The Public Health Science Journal. 2017;9(1):19–30.
Nalendrya I, Ilmi IMB, Arini F. Sosis Ikan Kembung (Rastrelliger Kanagurta L.) sebagai Pangan Sumber Omega 3. Jurnal Aplikasi Teknologi Pangan. 2016;5(3):71–5.
Cahyati AI, Nurrahman N, Aminah S. Sifat Kimia dan Fisik Engay Food Berbasis Ikan Kembung dengan Penambahan Kedelai Hitam. AGRITEKNO: Jurnal Teknologi Pertanian. 2022 Jan 29;11(1):9–17.
Hamid MN. Menggali Potensi Genetik Tanaman Kacang Bogor (Vigna subterranea (L.) Verdcourt). [Internet]. [Bogor]: IPB University; 2009 [cited 2025 Nov 18]. Available from: http://repository.ipb.ac.id/handle/123456789/19343
Surahman Y, Yuliawati Y, Setyono. Growth and Yield of Eight Bambara groundnut (Vigna subterranea L. Verdc.) Accessions From Bogor and Sukabumi. JURNAL AGRONIDA. 2023 Oct 31;9(2):103–12.
Yahaya D, Seidu OA, Tiesaah CH, Iddrisu MB. The role of soaking, steaming, and dehulling on the nutritional quality of Bambara groundnuts (Vigna subterranea (L) Verdc.). Front Sustain Food Syst [Internet]. 2022 Aug 12 [cited 2025 Nov 18];6. Available from: https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2022.887311/full
Wicaksono MG, Burahman H, Lestari YD. Assessment of Coronary Heart Disease Risk Among Medical Faculty Members Using The Jakarta Cardiovascular Score (JAKVAS). Journal of Health and Nutrition Research. 2025 Aug 1;4(2):616–25.
Karnjanapratum S, Kaewthong P, Indriani S, Petsong K, Takeungwongtrakul S. Characteristics and nutritional value of silkworm (Bombyx mori) pupae-fortified chicken bread spread. Sci Rep. 2022 Jan 27;12(1):1492.
Bienkiewicz G, Tokarczyk G, Biernacka P. Influence of Storage Time and Method of Smoking on the Content of EPA and DHA Acids and Lipid Quality of Atlantic Salmon (Salmo salar) Meat. International Journal of Food Science. 2022;2022(1):1218347.
Syakir AY. Karakteristik Kimia dan Sensori Tempe Kacang Bogor (Vigna subterranea) Berdasarkan Lama Perebusan Selama Pengolahan. [Internet]. [Bogor]: IPB University; 2019 [cited 2025 Nov 18]. Available from: http://repository.ipb.ac.id/handle/123456789/99191
Wiriyawattana P, Suwonsichon S, Suwonsichon T. Effects of drum drying on physical and antioxidant properties of riceberry flour. Agriculture and Natural Resources. 2018 Oct 1;52(5):445–50.
AOAC. Official Methods of Analysis, 17th ed. Washington, DC: Association of Official Analytical Chemists. Washington: DC: Association of Official Analytical Chemists; 2006.
Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant. Songklanakarin Journal of Science and Technology (SJST). 2004 Mar 1;26(2):211–9.
Kusuma IM, Veryanti PR, Chairunnisa B. Aktivitas Antioksidan dari Ekstrak Metanol Buah Kawista (Limonia acidissima) dengan Metode DPPH (1,1-difenil-2-pikrilhidrazil): Antioxidant Activity on Methanol Extract of Kawista (Limonia acidissima) Fruit with DPPH Method (1,1-diphenyl-2-picrylhydrazil). Sainstech Farma: Jurnal Ilmu Kefarmasian. 2020 Sept 10;13(2):60–5.
Tharaka THS, Arupala ALYH, Jayasinghe JMP, Abeyrathne EDNS. Development of a bread-spread using catla catla and mature flower buds of Rhizophora apiculata. SL J Food & Agric. 2016 Dec 29;2(2):29–38.
Karnjanapratum S, Kaewthong P, Indriani S, Petsong K, Takeungwongtrakul S. Characteristics and nutritional value of silkworm (Bombyx mori) pupae-fortified chicken bread spread. Sci Rep. 2022 Jan 27;12(1):1492.
Jayanti TAD, Sudarmanto A, Faqih MI. Cold Smoking Equipment Design Of Smoked Fish Products With Closed Circulation Using Temperature And Concentration Monitoring System Based On Arduino Uno. IOP Conf Ser: Mater Sci Eng. 2020 May;846(1):012025.
Tharaka THS, Arupala ALYH, Jayasinghe JMP, Abeyrathne EDNS. Development of a bread-spread using Catla catla and mature flower buds of Rhizophora apiculata. Sri Lanka Journal of Food and Agriculture. 2016;2(2):29–38.
Kiczorowska B, Samolińska W, Grela ER, Bik-Małodzińska M. Nutrient and Mineral Profile of Chosen Fresh and Smoked Fish. Nutrients. 2019 July;11(7):1448.
Maphosa Y, Jideani VA, Maphosa L. Bambara groundnut production, grain composition and nutritional value: opportunities for improvements. The Journal of Agricultural Science. 2022 Dec;160(6):448–58.
Abdualrahman MAY, Ma H, Yagoub AEA, Zhou C, Ali AO, Yang W. Nutritional value, protein quality and antioxidant activity of Sudanese sorghum-based kissra bread fortified with bambara groundnut (Voandzeia subterranea) seed flour. Journal of the Saudi Society of Agricultural Sciences. 2019 Jan 1;18(1):32–40.
Rahman N, Ariani A, Rakhman A. Beta-Carotene, Anthocyanin, Antioxidant Activity, and Microbiological Quality of Steamed Sponge Cakes for Alternative Post-Disaster Snack Food. Journal of Health and Nutrition Research. 2025 Apr 4;4(1):1–7.
Gao H, Yan B, Cheng F, Zhang S, Liao P, Li D, et al. The triglyceride-glucose index mediates associations between dietary inflammation index/Composite Dietary Antioxidant Index and cardiovascular disease incidence: insights from NHANES study. Exp Gerontol. 2025 July;206:112779.
Ren J, Sun X, Zhang Z, Pei H, Zhu S, Zhang Y, et al. Association between composite dietary antioxidant index and cardiovascular health: Results from two observational studies. Journal of Functional Foods. 2024 Sept 1;120:106372.
Setiawan G, Halim MC. Pengaruh Asam Lemak Omega-3 terhadap Penyakit Kardiovaskular. Cermin Dunia Kedokteran. 2022 Mar 1;49(3):160–3.
Published
How to Cite
Issue
Section
Copyright (c) 2025 Ross Mellyana Adistira, Sri Anna Marliyati, Budi Setiawan

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.









