Effect of Natural Antioxidant Supplementation of Rose Cider (Rosa damascena) on Oxidative Stress in High-Risk Pregnant Women: An Analysis of Malondialdehyde (MDA) Biomarkers

Ani Laila; Fatiyani  Alyensi; Riana  Pertiwi

doi:10.56303/jhnresearch.v4i2.442

Authors

Ani Laila Poltekkes Kemenkes Riau, Indonesia
Fatiyani Alyensi Poltekkes Kemenkes Riau, Indonesia
Riana Pertiwi Politeknik Kesehatan Siteba, Padang, Indonesia

Keywords:

High-Risk Pregnancies, Oxidative Stress, Natural Antioxidants, Malondialdehyde, Rose Juice, Rosa Damascena

Abstract

Pregnancy in high-risk mothers is often linked to increased oxidative stress, which can cause obstetric complications and impair fetal development. Malondialdehyde (MDA), a byproduct of lipid peroxidation, is a commonly used biomarker to assess oxidative stress levels. Nutritional interventions based on natural antioxidants are gaining attention as a strategy to reduce oxidative stress during pregnancy. Rose cider (Rosa damascena) contains phenolic compounds and flavonoids with antioxidant properties. This study aimed to evaluate the effect of rose cider supplementation on MDA levels in high-risk pregnant women. A quasi-experimental design with a pretest-posttest measurements was conducted involving 60 high-risk pregnant women randomly assigned to two groups: the intervention group received 250 ml of rose cider daily for 30 days, while the control group received a placebo. MDA levels were measured before and after the intervention using the thiobarbiturate (TBA) spectrophotometry method. Results showed a significant reduction in MDA levels in the intervention group compared to the control group (p < 0.01). The intervention group’s MDA decreased by 1.87 ± 0.45 μmol/L, whereas the control group’s reduction was only 0.42 ± 0.31 μmol/L. These findings demonstrate the effectiveness of antioxidant compounds in rose cider in lowering oxidative stress among high-risk pregnant women. This supports the potential of herbal-based supplements as preventive measures in managing high-risk pregnancies. Further research is recommended to explore the long-term effects and molecular mechanisms underlying this supplementation.

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References

Parrettini S, Caroli A, Torlone E. Nutrition and metabolic adaptations in physiological and complicated pregnancy: focus on obesity and gestational diabetes. Front Endocrinol (Lausanne). 2020;11:611929.

Bohn MK, Adeli K. Physiological and metabolic adaptations in pregnancy: importance of trimester-specific reference intervals to investigate maternal health and complications. Crit Rev Clin Lab Sci. 2022;59(2):76–92.

Abu-Raya B, Michalski C, Sadarangani M, Lavoie PM. Maternal immunological adaptation during normal pregnancy. Front Immunol. 2020;11:575197.

Green ES, Arck PC. Pathogenesis of preterm birth: bidirectional inflammation in mother and fetus. In: Seminars in immunopathology. Springer; 2020. p. 413–29.

Cuffe JSM, Xu ZC, Perkins A V. Biomarkers of oxidative stress in pregnancy complications. Biomark Med. 2017;11(3):295–306.

Duhig K, Chappell LC, Shennan AH. Oxidative stress in pregnancy and reproduction. Obstet Med. 2016;9(3):113–6.

Pisoschi AM, Pop A, Iordache F, Stanca L, Predoi G, Serban AI. Oxidative stress mitigation by antioxidants-an overview on their chemistry and influences on health status. Eur J Med Chem. 2021;209:112891.

Chaudhary P, Janmeda P, Docea AO, Yeskaliyeva B, Abdull Razis AF, Modu B, et al. Oxidative stress, free radicals and antioxidants: Potential crosstalk in the pathophysiology of human diseases. Front Chem. 2023;11:1158198.

Mas-Bargues C, Escriva C, Dromant M, Borras C, Vina J. Lipid peroxidation as measured by chromatographic determination of malondialdehyde. Human plasma reference values in health and disease. Arch Biochem Biophys. 2021;709:108941.

Prins JR, Schoots MH, Wessels JI, Campmans-Kuijpers MJE, Navis GJ, van Goor H, et al. The influence of the dietary exposome on oxidative stress in pregnancy complications. Mol Aspects Med. 2022;87:101098.

Wang F, Miao M, Xia H, Yang LG, Wang SK, Sun GJ. Antioxidant activities of aqueous extracts from 12 Chinese edible flowers in vitro and in vivo. Food Nutr Res. 2017;

Hamza RZ, Al-Yasi HM, Ali EF, Fawzy MA, Abdelkader TG, Galal TM. Chemical characterization of Taif rose (Rosa damascena Mill var. trigentipetala) waste methanolic extract and its hepatoprotective and antioxidant effects against cadmium chloride (CdCl2)-induced hepatotoxicity and potential anticancer activities against liver cancer cells (HepG2). Crystals. 2022;12(4):460.

Siedlecki SL. Quasi-experimental research designs. Clin Nurse Spec. 2020;34(5):198–202.

Rogers J, Revesz A. Experimental and quasi-experimental designs. In: The Routledge handbook of research methods in applied linguistics. Routledge; 2019. p. 133–43.

Salim A, Mackinnon A, Christensen H, Griffiths K. Comparison of data analysis strategies for intent-to-treat analysis in pre-test–post-test designs with substantial dropout rates. Psychiatry Res. 2008;160(3):335–45.

Drezner Z, Turel O, Zerom D. A modified Kolmogorov–Smirnov test for normality. Commun Stat Comput. 2010;39(4):693–704.

Alkadi H. A review on free radicals and antioxidants. Infect Disord Targets (Formerly Curr Drug Targets-Infectious Disord. 2020;20(1):16–26.

Ifeanyi OE. A review on free radicals and antioxidants. Int J Curr Res Med Sci. 2018;4(2):123–33.

Hapsari YI, Rozi F, Asyifa MNF, Putranegara S, Balqis SP. Edukasi dan konseling gizi kepada ibu hamil KEK. J Bina Desa. 2022;4(2):195–203.

Gupta M, Govindappagari S, Burwick RM. Pregnancy-associated atypical hemolytic uremic syndrome: a systematic review. Obstet Gynecol. 2020;135(1):46–58.

Casas R, Castro-Barquero S, Estruch R, Sacanella E. Nutrition and cardiovascular health. Int J Mol Sci. 2018;19(12):3988.

Bala R, Singh V, Rajender S, Singh K. Environment, lifestyle, and female infertility. Reprod Sci. 2021;28(3):617–38.