Evaluation of Antioxidant Activity, α-Glucosidase Inhibition, and Malondialdehyde Reduction of Reformulated Galohgor Nutraceuticals from Sundanese-Indonesia

Yayik Dwi  Balgis; Roosita Katrin; Riyadi Hadi; Fathimah Fathimah

doi:10.56303/jhnresearch.v4i1.362

Authors

Yayik Dwi Balgis Postgraduate in Nutrition Science, Department of Community Nutrition, IPB University, Bogor, Indonesia
Roosita Katrin Department of Community Nutrition, IPB University, Bogor, Indonesia
Riyadi Hadi Department of Community Nutrition, IPB University, Bogor, Indonesia
Fathimah Fathimah Nutritional Science Study Program, Universitas Darussalam Gontor, Ponorogo, Indonesia

Keywords:

Alfa Glucosidase Inhibitor, Antioxidant, Diabetes, Galohgor Nutraceutical, Malondialdehyde

Abstract

Galohgor nutraceutical, a traditional herbal formulation from Sundanese-Indonesia, has long been used for its health benefits, including antidiabetic potential. However, studies on α-glucosidase inhibition in its new formulation remain limited. This study aimed to assess the antioxidant activity, α-glucosidase inhibitory potential of the reformulated (F2) of Galohgor Nutraceutical, and its effect on malondialdehyde (MDA) levels. The methods used a completely randomized design (CRD). Antioxidant activity and α-glucosidase inhibitory effect were evaluated using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) method and α-glucosidase inhibition assay. MDA levels were analyzed in vivo using serum samples from mice treated with the reformulated (F2) of Galohgor Nutraceutical. The statistical analysis used was an independent t-test. The results the reformulated (F2) of Galohgor Nutraceutical extract exhibited antioxidant activity, with IC₅₀ values of 2904.06 ppm, while ascorbic acid, used as a positive control, had a significantly lower IC₅₀ value of 8.20 ppm. The α-glucosidase inhibitory activity of the reformulated (F2) extracts showed IC₅₀ values of 30.833,14 ppm, respectively, which were considerably higher than the positive control, acarbose (IC₅₀= 0.16±0.001 ppm). Administrating of the Galohgor extract at a dose of 5000 mg/kg body weight successfull reduced MDA levels. Although not statistically significant (p = 0.131), the observed reduction in MDA levels suggests a protective potential against oxidative stress. The reformulated (F2) of Galohgor Nutraceutical extract has potential as a natural antioxidant and antidiabetic agent

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